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KMID : 0923620140140020089
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Immune Network
2014 Volume.14 No. 2 p.89 ~ p.99
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Kinetics of IFN-? and IL-17 Production by CD4 and CD8 T Cells during Acute Graft-versus-Host Disease
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Ju Ji-Min
Lee Hak-Mo
Oh Keun-Hee
Lee Dong-Sup
Choi Eun-Young
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Abstract
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Graft-versus-host disease (GVHD) is a fatal complication that occurs after allogeneic hematopoietic stem cell trans-plantation. To understand the dynamics of CD4 and CD8 T cell production of IFN-? and IL-17 during GVHD progression, we established a GVHD model by transplanting T cell-de-pleted bone marrow (TCD-BM) and purified T cells from B6 mice into irradiated BALB.B, creating an MHC-matched but minor histocompatibility (H) antigen-mismatched trans-plantation (B6 → BALB.B GVHD). Transplantation-induced GVHD was confirmed by the presence of the appropriate compositional changes in the T cell compartments and innate immune cells in the blood and the systemic secretion of in-flammatory cytokines. Using this B6 → BALB.B GVHD mod-el, we showed that the production of IFN-? and IL-17 by CD4 T cells preceded that by CD8 T cells in the spleen, mesen-teric lymph node, liver, and lung in the BALB.B GVHD host, and Th1 differentiation predated Th17 differentiation in all organs during GVHD progression. Such changes in cytokine production were based on changes in cytokine gene ex-pression by the T cells at different time points during GVHD development. These results demonstrate that both IFN-? and IL-17 are produced by CD4 and CD8 T cells but with dif-ferent kinetics during GVHD progression.
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KEYWORD
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GVHD, B6 → BALB.B GVHD model, Kinetics of IFN-? and IL-17 production
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